Figure 3. Effect of increasing temperature (37°C – 43°C) for 1 h on the gene expressions of occludin (A) and ZO-1 (B) by Real-time PCR.

Cells were cultured for 24 h after 1 h heat exposure. Values were normalized to 37°C group (37°C set to 1). Results were reported as means ± SD. N = 3 per group. ** P<0.01 compared with 37°C group.