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Cells were cultured for 24(37°C group and 43°C group) or with PUFAs pre-incubation for 96 h. TJ proteins in the membrane fraction were shown (A): occludin (B), ZO-1 (C) and claudin-2 (D). Results were reported as means ± SD from 3 independent experiments. Values were normalized to β-actin. * P<0.05, ** P<0.01 compared with 37°C group. # P<0.05,## P<0.01 compared with 43°C group.
