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. 2013 Sep 16;8(9):e73571. doi: 10.1371/journal.pone.0073571

Figure 9. Effect of PUFAs pretreatment on the gene expressions of occludin (A) and ZO-1 (B) after heat stress by Real-time PCR.

Figure 9

After pre-incubation with PUFAs or not (37°C group and 43°C group) for 96 h, Caco-2 monolayers were harvested 24 hours after 1 h of heat exposure. Expression of mRNA was normalized with GAPDH mRNA expression. Values were normalized to 37°C group (37°C set to 1). Results were reported as means ± SD from 3 independent experiments. N = 3 per group.* P<0.05, ** P<0.01 compared with 43°C group.