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. 2013 Sep 16;8(9):e73923. doi: 10.1371/journal.pone.0073923

Figure 3. Activation of VcCheY3 and its mutants, measured through fluorescence quenching.

Figure 3

(a) Tryptophan quenching of VcCheY3 and its different mutants (indicated at top of the figure) using acetyl phosphate (acP) as substrate. (b) Plot of ΔI/I0 vs acP concentration (in mM) and corresponding Km values (both in graphical and numerical modes); (c) Tryptophan quenching of VcCheY3 and its different mutants (indicated at top of the figure) using BeF3 as substrate.