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Immunohistochemistry (peroxidase method with DAB colorization) for GLP-1 (1/500 dilution) in pancreata from vehicle-treated (a) or STZ-treated (c and d) rats. This antiserum recognizes GLP-17-37 and GLP-11-37) and may recognize proglucagon and MPGF, but not amidated forms of GLP-1 or glucagon. A large increase in GLP-1 immunoreactivity is seen in the STZ-treated islets corresponding to cells that stain for glucagon. Scale bar = 50 μm; 2 animals per group.
