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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Methods Mol Biol. 2013;1005:143–156. doi: 10.1007/978-1-62703-386-2_12

Figure 2.

Figure 2

A, Myocardial tissue homogenates (300 μg) are subjected to immunoprecipitation with AbGSC90, and subsequently subjected to SDS-PAGE and immunoblotted with anti-70 kDa and anti-GSH antibodies. B, Complex II is isolated from the bovine heart. Lane 1, native complex II (50 pmol, based on heme b) is subjected to SDS-PAGE and followed by immunoblotting with anti-GSH (upper panel) and anti-70 kDa antibodies (lower panel). Lane 2, native complex II pretreated with β-ME (1%) prior to SDS-PAGE. Lane 3, glutathionylated complex II. Lane 4, glutathionylated complex II pre-treated with β-ME (1%) prior to SDS-PAGE.