Figure 6.

Translocation of cPLA₂ in single alveoli. An alveolus was infused with 1,000 U/mL TNF-α (a–d) or with Ringer’s solution (e and f). Then the alveolus was stained for both the immunofluorescence of cPLA₂ (a, b, e) and the nuclear fluorescence of HOECHST 33324 (c and f). The alveolus was washed and imaged at low (a) and high (b–f) magnifications as indicated by the scale bars. Color code shows fluorescence intensities. Cell margins identified by bright-field microscopy are depicted by line sketches. Alv, alveolar lumen. Images for cPLA₂ and nuclear fluorescence were combined by image overlay (d, f). Nuclear outline is indicated by dotted line (d). Replicated 4 times.