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. 2013 Sep 16;8(9):e75313. doi: 10.1371/journal.pone.0075313

Figure 1. ULK1 knockdown increases melanin in MNT-1 cells.

Figure 1

(A) MNT-1 cells were treated with a control siRNA, two ULK1-specific siRNAs and a TYR-specific siRNA pool as a positive control for changes in melanin levels. Three days after the second knockdown cells were harvested and their melanin content was analysed by measuring melanin/protein values for each sample. Significant changes relative to control were determined by a one-way ANOVA test based on three individual experiments performed in duplicates. * p<0.05; ** p<0.01; *** p<0.001; data are presented as the mean of values normalized to control within each experiment. (B) Western blot analysis of the siRNA-treated MNT-1 cells verifying the knockdowns. Samples are loaded in duplicates. Tubulin is used as a loading control.