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. 2000 Apr 1;105(7):887–895. doi: 10.1172/JCI8688

Figure 1.

Figure 1

The del 62-64 V2R mutant is not expressed at the cell surface and is retained in the ER. (a and b) Nonpermeabilized cell immunofluorescence microscopy of COS-1 cells expressing the myc-tagged wild-type (WT) or myc-tagged del 62-64 V2R, respectively. (c) The same field of cells as in b stained with rhodamine-conjugated phalloidin to label actin filaments. (d and e) Permeabilized cell immunofluorescence microscopy of COS-1 cells expressing the myc-tagged WT or myc-tagged del 62-64 V2R, respectively. Bar = 50 μm. (f–h) Confocal immunofluorescence microscopy on permeabilized COS-1 cells expressing the myc-tagged del 62-64 V2R using the anti-myc antibody (f), an antibody directed against the ER-resident protein calreticulin (g). (h) Illustration of the superimposed localization of both proteins. Bar = 20 μm. Representative of 5 separate experiments.