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. 2000 Apr 1;105(7):887–895. doi: 10.1172/JCI8688

Figure 4.

Figure 4

Concentration-dependent increase of cell-surface del 62-64 V2R after SR121463A treatment. Cell-surface receptor expression was measured by FACS of HEK-293 cells stably expressing either del 62-64 (a) or WT (b) V2R. Cells were treated with the indicated concentrations of SR121463A. Representative of 3 separate experiments. (c) Dose-dependent effect of SR121463A treatment on cell-surface del 62-64 (filled triangles) and WT (filled squares) V2R expression as quantified by the mean cell-surface fluorescence intensity obtained by FACS. The 100% point was taken from cells exposed to a 10–5 M concentration of SR121463A, and the 0% point was taken from cells incubated without anti-myc antibody. There was no difference in cell-surface fluorescence intensity between untransfected HEK-293 cells incubated with both antibodies and HEK-293 cells expressing the del 62-64 V2R that were incubated without anti-myc antibody. There was no difference in cell-surface fluorescence intensity between untransfected HEK-293 cells incubated with both antibodies and HEK-293 cells expressing the del 62-64 V2R that were incubated without anti-myc antibody. The data are expressed as means ± SEM (n = 3).