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. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: J Immunol. 2011 May 4;186(12):6673–6682. doi: 10.4049/jimmunol.1002544

Figure 6.

Figure 6

Decreased Cdkn2c expression has opposite effects in B6.Sle2c1 sB cells and Pc B1a cells. A, Cdkn2c expression in sB cells stimulated with LPS. Values for B6.Sle2c1.Rec1a (square) and B6.Sle2c1.Rec1b (star) were obtained only at 72 h. B, IgM secretion by sB cells after 72 h stimulation with LPS. ELISA were performed on supernatants diluted 1:100. C, Cdkn2c expression in sB cells stimulated with anti-IgM. D, Cdkn2c expression in Pc B1a cells cultured for 72 h with or without LPS. E, IgM secretion by Pc B1a cells after 72 h stimulation with LPS. The X axis shows the supernatant dilutions at which the ELISA assay was performed. For A and C, Cdkn2c was normalized to unstimulated sB and Pc B1a cell B6 values. F. Representative CFSE dilution in splenic or Pc B220+ CD5+ (B1a) and B220+ CD5− (B2) cells after 72 h LPS stimulation performed in 5 mice per strain. The filled grey histograms indicate B6 and the open black histograms indicate B6.Sle2c1 values. G, Absolute numbers of proliferating Ki67+ sB cells and Pc B1a cells in B6, B6.Sle2c1, and B6.Sle2c1.Rec1a and B6.Sle2c1.Rec1b mice. In B, D, and G, graphs show individual mice and means plus SEM. Graphs in A, C, and E show means plus SEM, and the differences between B6 and B6.Sle2c1 values were significant at p < 0.05 at all time points or dilutions. Statistical significance in B, D and G was obtained with Dunnett's multiple comparison tests to B6 values. *: p < 0.05, and **: p < 0.01.