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. 2013 Jul 30;53(2):89–93. doi: 10.3164/jcbn.13-28

Fig. 2.

Fig. 2

Down-regulation of Mcl-1 by Mithramycin A or siRNA increases truncated Bid. (A) Bcl-2 family members [truncated Bid (tBid), Bim, Bak, Bax, Bcl-xL and Bcl-2] in DU145 and PC3 cells treated with dimethyl sulfoxide or various concentration of Mithramycin A (Mith) were evaluated by Western blot analysis. The results are representative of three independent experiments. (B) Cells were transiently transfected with either control siRNA (sicon) or siRNA specific to Mcl-1 (siMcl-1) for 72 h. Mcl-1, tBid and Bcl-XL proteins were analyzed by Western blot analysis.