Fig. 5.

Mitochondrial injury by exposing ethanol. Mitochondrial injury was measured by JC-1 after exposed-ethanol for 1 h. Green fluorescence (Ex. 485 nm, Em. 535 nm) and red fluorescence (Ex. 560 nm, Em. 595 nm) show mitochondrial injury and healthy mitochondria, respectively. Muted mitochondria were significantly increased under the condition of 5% (v/v) ethanol. (A) Confocal microscopic fluorescent images, (B) Red fluorescence intensity of JC-1, (C) Green fluorescence intensity of JC-1. The fluorescence intensity was measured by plate reader. n = 4, Error bar; SD.