Table 1.
Analysis of the five TAP gene polymorphisms.
| SNP | Allele specific probes | PCR product | Sequencing primer | Annealing temperature |
|---|---|---|---|---|
| TAP1 1341 | biotin-5′-TACCTGGTGCGAGGCCTATGTC | 150 bp | 5′-AAAAGCAGAGGCAGG | 64°C |
| 5′-AGCGGGCCAACTCCATGAAC | ||||
|
| ||||
| TAP1 2254 | 5′-CATGTGGCTATACCGTTC | 135 bp | 5′-TTGCTCTGCAGAGGTAG-3' | 52°C |
| biotin-5′-GGATAAGTACACACGGTTTC | ||||
|
| ||||
| TAP2 1135 | 5′-GAAGTCTGTCGCTATAAAGAG | 124 bp | 5′-CGCCTTGTACCTGCT-3′ | 52°C |
| biotin-5′-GGCTCCTTTCACAACCAC | ||||
|
| ||||
| TAP2 1693 | biotin-5′-GCACAGGTGGTTTCAGTTGG | 96 bp | 5′-GCTCTGCAGCCCATA-3′ | 58°C |
| 5′-CTTATCATCTTCGCAGCTCTGC | ||||
|
| ||||
| TAP2 1993 | 5′-TCTTTCTGAGGCACTGTGATC | 283 bp | 5′-TGATTGCTCACAGGCT-3′ | 54°C |
| biotin-5′-GGAAACTCAAAGCAGGAAC | ||||
SNP: single nucleotide polymorphism.
PCR: polymerase chain reaction.
TAP: transporter associated with antigen processing.