Table I.
Analysis of hematopoiesis in long term culture of caspase-1 –/– mouse bone marrow cells: weekly confluence of adherent cell layer (%).
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | |
|---|---|---|---|---|---|
| Caspase 1 –/v | 32.5±6.1 (n=4) | 76.9±6.9 (n=4) | 78.8±3.2 (n=4) | 81.3±1.4 (n=4) | 80.6±1.2 (n=4) |
| Caspase 1 +/+ | 29.4±3.1 (n=4) | 75.0±4.1 (n=4) | 81.3±1.4 (n=4) | 80.6±1.3 (n=4) | 80.0±0.0 (n=4) |
| p-value | 0.3990 | 0.6559 | 0.2070 | 0.5370 | 0.3910 |
| Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | |
| Caspase 1 –/– | 90.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) |
| Caspase 1 +/+ | 90.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) |
| p-value | 1 | 1 | 1 | 1 | 1 |
| Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | |
| Caspase 1 –/– | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) |
| Caspase 1 +/+ | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) |
| p-value | 1 | 1 | 1 | 1 | 1 |
| Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | |
| Caspase 1 –/– | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) |
| Caspase 1 +/+ | 95.0±0.0 (n=4) | 95.0±0.0 (n=3) | 95.0±0.0 (n=3) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) |
| p-value | 1 | 1 | 1 | 1 | 1 |
| Week 21 | Week 22 | Week 23 | Week 24 | Week 25 | |
| Caspase 1 –/– | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) |
| Caspase 1 +/+ | 95.0±0.0 (n=1) | 95.0±0.0 (n=1) | 95.0±0.0 (n=1) | 95.0±0.0 (n=1) | 95.0±0.0 (n=1) |
| p-value | 1 | 1 | 1 | 1 | 1 |
| Week 26 | Week 27 | Week 28 | Week 29 | Week 30 | |
| Caspase 1 –/– | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) | 95.0±0.0 (n=4) |
| Caspase 1 +/+ | 95.0±0.0 (n=1) | CT | CT | CT | CT |
| p-value | 1 | ||||
| Week 31 | Week 32 | Week 33 | Week 34 | Week 35 | |
| Caspase 1 –/– | 95.0±0.0 (n=4) | 95.0±0.0 (n=3) | 95.0±0.0 (n=3) | 95.0±0.0 (n=3) | 95.0±0.0 (n=3) |
| Week 36 | Week 37 | Week 38 | Week 39 | Week 40 | |
| Caspase 1 –/– | 95.0±0.0 (n=3) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) |
| Week 41 | Week 42 | Week 43 | Week 44 | Week 45 | |
| Caspase 1 –/– | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) |
| Week 46 | Week 47 | Week 48 | Week 49 | Week 50 | |
| Caspase 1 –/– | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) | 95.0±0.0 (n=2) |
| Week 51 | |||||
| Caspase 1 –/– | 95.0±0.0 (n=2) |
Data are summarized as mean±standard deviation, n is the number of mice used, and p-values were calculated with the two-sided two-sample t-test. Significant p-values are shown in bold. Due to a lack of hematopoietic activity beginning at week 27, the caspase-1 +/+ LTBMCs were terminated (CT).