Table II.
Analysis of hematopoiesis in long term cultures of caspase-1 –/– mouse bone marrow cells: weekly cobblestone numbers.
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | |
|---|---|---|---|---|---|
| Caspase-1 –/– | 215.8±75.2 (n=4) | 1478.0±227.3 (n=4) | 1072.5±263.2 (n=4) | 1149.9±172.6 (n=4) | 1391.9±264.5 (n=4) |
| Caspase-1 +/+ | 161.0±51.3 (n=4) | 1513.5±345.8 n=4) | 1211.0±351.9 (n=4) | 520.8±132.5 (n=4) | 964.5±422.2 (n=4) |
| p-value | 0.2743 | 0.8694 | 0.5517 | 0.0012 | 0.1371 |
| Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | |
| Caspase-1 –/– | 1507.3±197.0 (n=4) | 1597.5±156.5 (n=4) | 1469.0±167.3 (n=4) | 1320.0±36.4 (n=4) | 1693.0±321.0 (n=4) |
| Caspase-1 +/+ | 702.5±159.6 (n=4) | 527.5±151.7 (n=4) | 439.0±104.8 (n=4) | 357.5±114.9 (n=4) | 524.5±168.3 (n=4) |
| p-value | 0.0007 | <0.0001 | <0.0001 | <0.0001 | 0.0007 |
| Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | |
| Caspase-1 –/– | 1566.0±348.9 (n=4) | 1479.5±255.4 (n=4) | 1521.0±121.2 (n=4) | 1318.5±219.0 (n=4) | 1182.5±346.0 (n=4) |
| Caspase-1 +/+ | 327.5±67.4 (n=4) | 387.0±157.7 (n=4) | 307.5±250.2 (n=4) | 30.5±26.6 (n=4) | 51.8±100.2 (n=4) |
| p-value | 0.0048 | 0.0003 | 0.0001 | <0.0001 | 0.0008 |
| Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | |
| Caspase-1 –/– | 1075.0±425.8(n=4) | 1125.8±374.7(n=4) | 1249.0±545.6(n=4) | 948.0±392.0 (n=4) | 822.0±429.9 (n=4) |
| Caspase-1 +/+ | 159.0±318.0 (n=4) | 55.3±95.8 (n=3) | 0.0±0.0 (n=3) | 0.0±0.0 (n=2) | 0.0±0.0 (n=2) |
| p-value | 0.0137 | 0.0052 | 0.0196 | 0.0169 | 0.0315 |
| Week 21 | Week 22 | Week 23 | Week 24 | Week 25 | |
| Caspase-1 –/– | 979.8±220.3 (n=4) | 487.0±105.0 (n=4) | 461.3±222.7 (n=4) | 434.4±405.1 (n=4) | 355.5±415.8 (n=4) |
| Caspase-1 +/+ | 0.0±0.0 (n=2) | 0.0±0.0 (n=1) | 0.0±0.0 (n=1) | 0.0±0.0 (n=1) | 0.0±0.0. (n=1) |
| p-value | 0.0030 | ||||
| Week 26 | Week 27 | Week 28 | Week 29 | Week 30 | |
| Caspase-1 –/– | 405.0±469.9 (n=4) | 308.0±346.2 (n=4) | 277.0±346.2 (n=4) | 232.5±341.9 (n=4) | 210.3±291.1 (n=4) |
| Caspase-1 +/+ | 0.0±0.0 (n=1) | CT | CT | CT | CT |
| Week 31 | Week 32 | Week 33 | Week 34 | Week 35 | |
| Caspase-1 –/– | 190.5±243.7 (n=4) | 180.5±244.1 (n=4) | 227.3±279.9 (n=3) | 210.0±195.2 (n=3) | 96.7±90.0 (n=3) |
| Week 36 | Week 37 | Week 38 | Week 39 | Week 40 | |
| Caspase-1 –/– | 53.7±46.9 (n=3) | 67.3±108.1 (n=3) | 60.0±73.5 (n=2) | 66.0±93.3 (n=2) | 57.0±80.6 (n=2) |
| Week 41 | Week 42 | Week 43 | Week 44 | Week 45 | |
| Caspase-1 –/– | 48.0±67.9 (n=2) | 78.0±110.3 (n=2) | 54.5±77.1 (n=2) | 54.0±76.4 (n=2) | 62.0±87.7 (n=2) |
| Week 46 | Week 47 | Week 48 | Week 49 | Week 50 | |
| Caspase-1 –/– | 16.5±23.3 (n=2) | 12.5±17.7 (n=2) | 9.0±12.7 (n=2) | 4.5±6.4 (n=2) | 2.5±3.5 (n=2) |
| Week 51 | |||||
| Caspase-1 –/– | 0.0±0.0 (n=2) |
Data are summarized as mean±standard deviation, n is the number of mice used, and p-values were calculated with the two-sided two-sample t-test. Significant p-values are shown in bold. Due to a lack of hematopoiesis by the caspase-1 +/+ LTBMCs beginning at week 27, the cultures were terminated (CT).