Fig. 1.

Location of the GFP construct and a detailed view of the some of its features. The GFP construct is situated in the 3′ untranslated region of the Sfpi1 gene, located on mouse chromosome 2. The Sfpi1 gene is located in the AML minimal deleted region (mdr) between D2Mit126 and D2Mit185. Exons of the gene are denoted as boxes with the coding region in grey and introns as a black line, where exon 5 is specifically denoted. The direction of transcription is marked by arrows. Three primer binding sites forward A, reverse B and forward C are indicated. Stop: Stop codon, IRES: internal ribosome entry site, Neo: neomycin cassette.