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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: Methods. 2013 May 21;61(3):287–298. doi: 10.1016/j.ymeth.2013.05.004

Figure 5.

Figure 5

The diagram depicts how Normalized Group Area Ratio (NGAR) is calculated for a peptide with five MRM transitions over three samples three samples. The NGAR algorithm takes the peak areas for each transition within a run and calculates the ratio of the area of a transition to the area of the first transition for the corresponding group. It then divides by the average of this ratio for all samples (for a given transition). The net result is a NGAR that the reported value should be close to 1.0 if the ratio of a transition to the first is constant across the samples.