Table 1.
Primer set | Sample name | Match barcode | Passed initial processing | Contains frame-shift | Passed Frame-Bot | Avg. length |
---|---|---|---|---|---|---|
dxnA1/dfdA1 | Rhizosphere | 2,319 | 2,095 | 456 | 641 | 389 |
KS | 2,844 | 2,521 | 451 | 690 | 375 | |
Environmental sample total | 5,163 | 4,616 | 907 | 1,331 | ||
MC | 1,247 | 1,204 | 626 | 1,204 | 450 | |
carAa | Rhizosphere | 720 | 543 | 128 | 193 | 460 |
KS | 673 | 594 | 194 | 339 | 465 | |
Environmental sample total | 1,393 | 1,137 | 322 | 532 | ||
MC | 612 | 501 | 340 | 500 | 476 |
A mock community (MC) was composed of the strains used in validation of primer specificity and yielded the correct sequences and are not described further.