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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Mol Cell. 2013 Jun 6;51(2):236–248. doi: 10.1016/j.molcel.2013.05.003

Figure 5.

Figure 5

Nrf2 is stabilized by fumarate and helps mitigate ROS.

(A) Western blot for Nrf2 and tubulin protein and RT-PCR of Nrf2 target genes from cv and FH-FLAG UOK262 cells. (B) Western blot for Nrf2 and tubulin protein and RT-PCR of Nrf2 target genes from UOK262 cells expressing NS and OGDH shRNAs. (C) Western blot for Nrf2 and tubulin protein and RT-PCR of Nrf2 target genes from UOK262 cells expressing NS and SDHA shRNAs. (D) Western blot for Nrf2 and tubulin protein and RT-PCR of Nrf2 target genes from UOK262 cells treated with mock treatment, 1 μM TPP, and 1 μM MVE. (E) Western blot for HIF1α, Nrf2, and tubulin protein in UOK262 cells expressing NS or Nrf2 shRNAs. (F) Relative intracellular ROS level was determined by DCFH fluorescence in UOK262 cells expressing NS or Nrf2 shRNAs. (G) The proliferative effects of Nrf2 knockdown were measured after 48 hours and 96 hours. In (A–D, F, and G) the quantified values denote mean + s.e.m. n = 3. *P < 0.05; **P < 0.01.