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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Mol Cell. 2013 Jun 6;51(2):236–248. doi: 10.1016/j.molcel.2013.05.003

Figure 6.

Figure 6

ROS inhibit histone demethylation.

(A) Representative western blot for histone methylation markers and H4 protein loading control from nuclear extracts of UOK262 cells expressing cv and FH-FLAG plasmids. (B) Densitometric quantification of histone methylation markers relative to H4 loading control of UOK262 cv and FH-FLAG cells. (C) Measurement of demethylase activity of JMJD2A in vitro in the presence of fumarate, succinate, and N-oxalylglycine (NOG). (D) Measurement of demethylase activity of JMJD2A in vitro in the presence H2O2. (E) Measurement of synergistic inhibition of JMJD2A in vitro in the presence or absence of 1 mM fumarate and various doses of H2O2. Values are relative to activity of mock or fumarate treated cells without H2O2. In (B–E) the values denote mean + s.e.m. n = 3. *P < 0.05; **P < 0.01.