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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: J Immunol. 2013 Aug 16;191(6):3082–3089. doi: 10.4049/jimmunol.1300830

Figure 1. CD28-independent inhibition of T cell proliferation by CTLA4Ig in vitro.

Figure 1

CFSE labeled splenocytes were co-cultured with bone marrow derived macrophages and activated with PMA and ionomycin. Cells were harvested and stained with α-CD4 and proliferation determined by flow cytometry after 96 hours of culture. Where indicated, CTLA4Ig (10 μg/ml) and/or L-NMMA (100 μM) were included at the start of the culture. A) Representative CFSE dye dilution histograms of wild type splenocytes stimulated with PMA and ionomycin alone or in combination with BMDM and/or CTLA4Ig as indicated. The percentage of cells that have undergone at least one cell division is indicated. B) Splenocytes from wild type or CD28-deficient mice were co-cultured with wild type BMDM. Presented are the combined results from 6 independent experiments C) Splenocytes from wild type or NOS2 deficient mice were co-cultured with BMDM from wild type or NOS2 deficient mice. Presented are the combined results from 3 independent experiments. D) Splenocytes from wild type or CD80/CD86-double deficient mice were co-cultured with BMDM from wild type or CD80/CD86-double deficient mice. Presented are the combined results from 7 independent experiments. * = p<0.05, ** = p<0.01 by 2-tailed paired T-test