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. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: J Neurochem. 2013 May 13;125(6):869–884. doi: 10.1111/jnc.12255

Effects of long chain PUFA on differentiation of NSCs. NSCs were treated with 1 μM long chain PUFA bound to 0.05% BSA for 7 days and subjected to immunofluorescence and western blot analyses. NSCs were stained for MAP2 (green, mature neuron marker), Tuj-1 (green, early neuron marker), GFAP (red, glia marker) and nuclei (blue, DAPI), and visualized by fluorescence microscopy (A). The percentage of Tuj-1 and GFAP positive cells was evaluated using MetaMorph software (B). Western blot analysis was performed for MAP2, Tuj-1 and GFAP (C) and quantified by densitometry (D). The data are expressed as the mean ± SD of triplicates or quadruplicates, representing three to four independent experiments. ^a–c, Bars with different letter superscripts are significantly different (Bonferroni’s multiple comparison test, p<0.01).

Effects of long chain PUFA on differentiation of NSCs. NSCs were treated with 1 μM long chain PUFA bound to 0.05% BSA for 7 days and subjected to immunofluorescence and western blot analyses. NSCs were stained for MAP2 (green, mature neuron marker), Tuj-1 (green, early neuron marker), GFAP (red, glia marker) and nuclei (blue, DAPI), and visualized by fluorescence microscopy (A). The percentage of Tuj-1 and GFAP positive cells was evaluated using MetaMorph software (B). Western blot analysis was performed for MAP2, Tuj-1 and GFAP (C) and quantified by densitometry (D). The data are expressed as the mean ± SD of triplicates or quadruplicates, representing three to four independent experiments. ^a–c, Bars with different letter superscripts are significantly different (Bonferroni’s multiple comparison test, p<0.01).