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. Author manuscript; available in PMC: 2013 Sep 28.
Published in final edited form as: Cell. 2013 Mar 28;153(1):56–69. doi: 10.1016/j.cell.2013.03.004

Figure 2. Evidence of Transient Acetylation of Histone H3 Only during the Oxidative Phase of the Yeast Metabolic Cycle.

Figure 2

(A) Periodic fluctuation in oxygen levels in a chemostat growing prototrophic yeast. The yeast metabolic cycle (YMC) is roughly 5 hours in duration and defined by sequentially repeating oxidative (Ox), reductive building (RB), and reductive charging (RC) metabolic phases (adapted from Tu et al., 2005).

(B) Quantitative measurement of acetyl-CoA levels over the YMC reveal elevated levels of the metabolite during the Ox phase of the YMC. Western blot measurements of H3K9 acetylation over the YMC reveal dynamic acetylation temporally correlate with the peak abundance of acetyl-CoA.

(C) ChIP-seq analysis of H3K9 acetylation on the promoter of the gene encoding the RPS7B ribosomal protein reveals modification limited to the Ox phase of the YMC.

(D) Transcript abundance of the RPS7B mRNA peaks during the Ox phase of the YMC precisely when acetyl-CoA levels are of highest abundance and when the promoter of the RPS7B gene is modified by H3K9 acetylation.