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. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: Adv Funct Mater. 2013 Feb 13;23(27):3404–3412. doi: 10.1002/adfm.201202926

Figure 6.

Figure 6

hMVECs co-cultured with human mesenchymal stem cells (hMSCs) in the microchannel platform. (A) Schematic of the seeding protocol. hMVECS were seeded first on the patterned scaffold surface. After two days of static culture, the microchannels were enclosed and hMSCs were seeded in the platform bulk space. (B) After the bonding step and one day of dynamic culture, the hMVECs (red) were observed in the microchannel space and the hMSCs (blue) were observed in the bulk space. After seven days of dynamic culture the hMVECs proliferated in the microchannel space, while hMSCs proliferated in the platform bulk (scale bars = 300 μm). (C) Higher magnification of a microchannel after seven days of dynamic culture shows hMVECs lining the microchannel while hMSCs populate the space around the microchannel (scale bar = 50 μm).