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. 2013 Oct 1;140(19):4110–4115. doi: 10.1242/dev.094615

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Validation of the reporter cell line. (A) qRT-PCR analysis during retinoic acid differentiation of ESCs showing neomycin and Gata6 expression. (B) Eed expression analysis of clones containing scrambled or shRNA targeting Eed. (C) Ring1B expression analysis of clones containing scrambled or shRNA targeting Ring1B. (D) Neomycin and Gata6 expression of the clones shown in B. (E) Neomycin and Gata6 expression of the clones shown in C. Error bars represent 95% confidence intervals of three technical replicates. KD, knockdown.