Table 4.

Methodologies used to overproduce drugs using engineered proteomics approach.

Strains	Drugs	Approach	Methodologies
S. venezuelae	Pikromycin	Proteomics facilitated reverse engineering to enhance secondary metabolite production	Overexpression of the metK gene encoding SAM synthetase
S. griseus IFO13189 and S. griseoflavus FERM1805	Spectinomycin and bicozamycin	Proteomics facilitated reverse engineering to enhance secondary metabolite production	Exogenous feeding of S-Adenosyl methionine (SAM) results in enhanced production
S. sp. FR-008, S. avermitilis, S. coelicolor A3 (2), S. lividans TK23, and S. antibioticus ATCC11891	Candicin D, avermectin, actinorhodin, and oleandomycin	Proteomics facilitated reverse engineering to enhance secondary metabolite production	Coexpression of metK or exogenous feeding of SAM enhanced antibiotic production
S. avermitilis	Avermectins	Proteomics facilitated reverse engineering to enhance secondary metabolite production	Overexpression of mutant library of sigma factor σhrdB  enhanced antibiotic production
S. peucetius ATCC 27952	Doxorubicin	Proteomics facilitated reverse engineering to enhance secondary metabolite production	Overexpression of efflux protein DrrA enhanced antibiotic production
S. lividans TK24	Actinorhodin	Ribosome engineering to enhance secondary metabolite production	Resistance against streptomycin causes production of pigmented antibiotic actinorhodin not produced in normal laboratory conditions
S. chattanoogensis	Fredericamycin	Ribosome engineering to enhance secondary metabolite production	Resistance against streptomycin causes enhanced production
S. lividans 66	Actinorhodin, undecylprodigiosin, and calcium dependent antibiotics	Ribosome engineering to enhance secondary metabolite production	By introducing rifampicin mutations into the rpoB (encoding the RNA polymerase subunit) gene
S. coelicolor A3 (2)	Actinorhodin	Ribosome engineering to enhance secondary metabolite production	By introducing double and triple mutations using gentamicin rifampicin and streptomycin increases actinorhodin production
S. coelicolor A3 (2)	Actinorhodin	Ribosome engineering to enhance secondary metabolite production	Enhanced expression of ribosome recycling factor by mutation increases production
S. avermitilis	Avermectin	Ribosome engineering to enhance secondary metabolite production	Overexpression of ribosome recycling factor increases production
S. coelicolor A3 (2)	Chloramphenicol and congocidine	Ribosome engineering to enhance secondary metabolite production	Introducing rpsL and rpoB mutations enhanced chloramphenicol and congocidine production
Streptomycetes	Antibiotics	Ribosome engineering to enhance secondary metabolite production	Introducing mutations in rsmG gene encoding for 16S rRNA methyltransferase