Figure 3.

BDMC increased Sirt1 in transcriptional and translational levels in WI38 cells. (a) Dose response: cells were treated with different doses of BDMC (5–40 μM) for 48 h. (b) Time response: cells were exposed to 20 μM of BDMC for different duration (12–72 h). Then Sirt1 expression was measured by Western blot assay. (c) Cells were treated with different doses of BDMC (5–40 μM) for 24 h; then total RNA was extracted, and reversed transcription, and real-time PCR were performed to assess Sirt1 gene level. Data were presented as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01 compared with control group.