TABLE 1.
Crystallographic data collection and refinement1
| ΔN1GspE-6aa-Hcp1 | ΔN1GspE-8aa-Hcp1 | |
|---|---|---|
| Data Collection | ||
| Space Group | P 42 21 2 | P 2 21 21 |
| Unit cell dimensions a,b,c (Å) | 205.1, 205.1, 235.0 | 112.5, 132.9, 142.8 |
| Mosaicity (°) | 0.53 | 0.23 |
| Resolution range (Å) | 60.0–7.6 (7.87-7.6) | 38.7, 4.09 (4.31-4.09) |
| Total no. of reflections | 45,135 | 111,225 |
| Unique reflections | 6,587 | 17,346 |
| Average redundancy | 6.9 (7.0) | 6.4 (6.1) |
| % completeness | 99.6 (99.9) | 99.2 (97.5) |
| Rmerge | 0.25 (>1) | 0.083 (0.959) |
| <I/σ(I)> | 10.9 (2.0) | 7.1 (0.8) |
| Refinement | ||
| Resolution (Å) | 50.0–7.6 (7.9-7.6) | 38.7–4.2 (4.38-4.2) |
| No. reflections | 6265 (432) | 15196 (1014) |
| Rwork & Rfree | 0.363/0.368 | 0.341 & 0.371 |
| Number of protein chains per a.u. | Six ΔN1EpsE-6aa-Hcp1 chains. Total Mw 383 kDa. | Three ΔN1EpsE-8aa-Hcp1 chains. Total Mw 193 kDa. |
1
Numbers in parentheses refer to outer resolution shell