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. Author manuscript; available in PMC: 2014 May 30.
Published in final edited form as: Cell Rep. 2013 May 16;3(5):1640–1650. doi: 10.1016/j.celrep.2013.04.016

Figure 5. Senp1 Is Required to Remove Terminal Sumo1 Moieties on Sumo2/3 Chains.

Figure 5

Immunoblots of SDS lysates from M1 MEFs infected with HA-Sumo2 retroviral expression vector (lanes 2, 4, 6, and 8) or empty HA vector (lanes 1, 3, 5, and 7). Direct analysis with anti-HA (lane 2) or anti-HA immunoblotting of anti-HA immunoprecipitates (lane 4) shows HA-Sumo2/3 conjugates across the full range of molecular masses. Analysis of anti-HA immunoprecipitates with anti-Sumo1 (lane 8) reveals only HMM species (bracket), suggesting that these represent long poly-Sumo2/3 chains capped with Sumo1.