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. Author manuscript; available in PMC: 2014 May 30.
Published in final edited form as: Cell Rep. 2013 May 16;3(5):1640–1650. doi: 10.1016/j.celrep.2013.04.016

Figure 6. Sumo1 Heterozygosity Rescues Senp1 Mutant Defects.

Figure 6

(A) Immunoblots of SDS lysates from E12.5 embryos detected with anti-Sumo1. Lanes 1–4 show WT, Sumo1 heterozygote (Sumo1+/−), Senp1M1/M1 (M1), and Senp1M1/M1 Sumo1+/− (M1 Sumo1+/−); lanes 5–8 show WT, Sumo1+/−, Senp1Gt/Gt (Gt), and Senp1Gt/Gt Sumo1+/− (Gt Sumo1+/−). Sumo1 heterozygosity significantly lowers accumulation of Sumo1 conjugates in mutant embryos (compare lanes 3 and 4 and lanes 7 and 8). Molecular weights in kilodaltons (kDa) are shown to the left of each panel. Lower panels show anti-actin immunoblots to control for equal loading.

(B) Immunoblots of SDS lysates from E12.5 embryos detected with anti-Sumo2/3. The same samples were analyzed and loaded in the same order as in (A).

(C) Left panel shows an E14.5 Senp1M1/M1 Sumo1+/− embryo that was viable when dissected and a dead Senp1M1/M1 littermate. Right panel shows a rescued E15.5 Senp1Gt/Gt Sumo1+/− embryo and a dead Senp1Gt/Gt littermate.