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. 2013 Sep 17;33(5):e00068. doi: 10.1042/BSR20130069

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Scatchard plots of HS binding affinities obtained by SPR. wtCXCL8 (■ K_d=1 μM), CXCL8(Δ6E70KN71K) (○ K_d=265nM) and CXCL8(Δ6F17KF21KE70KN71K) (● K_d=65 nM). (B) Guanidine-induced unfolding curves of wtCXCL8 and CXCL8(Δ6E70KN71K). Denaturation was monitored by fluorescence wavelength shift and characterized by the transition midpoint of unfolding T_50% [x₀ in Eqn. (2)] and by the relative cooperativity [dx in Eqn. (2)].