Table 1. The three classes of designed CXCL8 mutants including a gradation of the type of mutation.

+, strong mutation; ~, light mutation.

CXCL8 mutant		Type/location of mutation
CXCL8(F21R)	1.class of mutants	~/ loop
CXCL8(F21K)		+/ loop
CXCL8(F17K)		+/ loop
CXCL8(F17RF21R)		~~/ loop
CXCL8(F17KF21R)		+~/ loop
CXCL8(F17RF21K)		~+/ loop
CXCL8(F17KF21K)		++/ loop
CXCL8(Δ6E70R)	2.class of mutants	~/ -ELR, α-helix
CXCL8(Δ6E70KN71K)		++/ -ELR, α-helix
CXCL8(Δ6F17RF21RE70K)	3.class of mutants	~~+/ -ELR, loop, α-helix
CXCL8(Δ6F17RF21RN71K)		~~+/ -ELR, loop, α-helix
CXCL8(Δ6F17RF21RE70KN71R)		~~+~/ -ELR, loop, α-helix
CXCL8(Δ6F17RF21RE70RN71K)		~~~+/ -ELR, loop, α-helix
CXCL8(Δ6F17KF21KE70KN71K)		++++/ -ELR, loop, α-helix