Table 2. K_d of the 1.class CXCL8 mutants, as determined by IFT, compared with wtCXCL8.

Ligands were LMWH and unfractionated HS

wtCXCL8 or mutant	Kd (×10−6 M)/LMWH	Kd (×10−6 M)/unfractionated HS
wtCXCL8	2.28±0.51	0.46±0.072
CXCL8(F21R)	5.38±0.54	0.24±0.032
CXCL8(F21K)	2.92±0.27	1.72±0.22
CXCL8(F17K)	3.20±0.23	1.45±0.16
CXCL8(F17RF21R)	4.00±0.53	0.88±0.07
CXCL8(F17KF21R)	4.49±0.41	0.92±0.12
CXCL8(F17RF21K)	2.88±0.29	1.70±0.15
CXCL8(Δ3F17KF21K)	n.d.	n.d.