. 2013 Sep 17;33(5):e00068. doi: 10.1042/BSR20130069

Table 4. K_d and relative saturation of HS-binding sites of the 3.class CXCL8 mutants, as determined by IFTs, compared with wtCXCL8.

Ligand: unfractionated HS. A high degree of fluorescence quenching refers to tighter binding of the HS ligand leading to more efficient deactivation of the emission.

wtCXCL8 or mutant	K_d (×10⁻⁶ M)/unfractionated HS	Final degree of fluorescence quenching (max. ΔF/F₀) in%)
wtCXCL8	0.46±0.072	15
CXCL8(Δ6F17RF21RE70K)	0.103±0.0152	27
CXCL8(Δ6F17RF21RE70RN71K)	0.107±0.0091	22
CXCL8(Δ6F17RF21RE70KN71R)	0.237±0.0614	24
CXCL8(Δ6F17RF21RN71K)	0.209±0.0145	32
CXCL8(Δ6F17KF21KE70KN71K)	0.097±0.0081	38

Table 4. Kd and relative saturation of HS-binding sites of the 3.class CXCL8 mutants, as determined by IFTs, compared with wtCXCL8.

Table 4. K_d and relative saturation of HS-binding sites of the 3.class CXCL8 mutants, as determined by IFTs, compared with wtCXCL8.