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. 2013 Jun 12;2(4):141–145. doi: 10.1002/open.201300018

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© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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A) Amperometric responses of IL-6 in undiluted calf serum at −0.2 V vs. Ag/AgCl developed by injecting 1 mm HQ and 0.1 mm H₂O₂ after capturing analyte-Ab₂-MB_tosyl-HRP bioconjugates on the sensors in the microfluidic device. Inset shows lowest level and control on expanded current scale. B) Corresponding calibration curve y=27.9+32.7 log(x); R=0.99.