Table 1.
Plasmid | mE-box 1 | mE-box 2 |
---|---|---|
E-box sequence | ATCCAGCTGTCC | CTTCACCTGGGC |
Mutated sequence | ATCCAGCTAGCC | CGGTACCTGGGC |
Forward primer | 5′CAATGAAGAAAAATCCAGCTAGCCCTTCCAAGGGGAGGTATC | 5′CGCCTAGCCCCCAGCGGTACCTGGGCCCCTCCC |
Reverse primer | 5′GATACCTCCCCTTGGAAGGGCTAGCTGGATTTTTCTTCATTG | 5′GGGAGGGGCCCAGGTACCGCTGGGGGCTAGGCG |
New restriction digest site | Nhe1 | Kpn1 |