Table 1.

Mutation of E-boxes in αENaC promoter-luciferase construct.

Plasmid	mE-box 1	mE-box 2
E-box sequence	ATCCAGCTGTCC	CTTCACCTGGGC
Mutated sequence	ATCCAGCTAGCC	CGGTACCTGGGC
Forward primer	5′CAATGAAGAAAAATCCAGCTAGCCCTTCCAAGGGGAGGTATC	5′CGCCTAGCCCCCAGCGGTACCTGGGCCCCTCCC
Reverse primer	5′GATACCTCCCCTTGGAAGGGCTAGCTGGATTTTTCTTCATTG	5′GGGAGGGGCCCAGGTACCGCTGGGGGCTAGGCG
New restriction digest site	Nhe1	Kpn1