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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Am J Hematol. 2013 Jun 20;88(8):670–676. doi: 10.1002/ajh.23478

Figure 2. Specificity of the ELISA for GCD59.

Figure 2

Sandwich ELISA of affinity purified GCD59 before and after immunoprecipitation (IP) with increasing concentrations of goat polyclonal anti-CD59 antibody. The insets show the western blot (WB) analysis for total (red fluorescence) and GCD59 (green fluorescence) of the sample before (inset at 0 antibody concentration) and after immunopreciptiation (IP) with 12 μg/ml antibody (S= WB of the supernatant after IP; E = WB of eluate from agarose beads carrying the antibody-CD59 complex).