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. 2013 May 24;43(2):383–393. doi: 10.3892/ijo.2013.1953

Figure 1.

Figure 1

Confirmation that HPV16-negative cell lines expressed HPV16 E6 and E7 oncogenes only after transfection. We confirmed the E6E7 DNA amplifications both YD8 and YD10B cells expressed E6 and E7 oncogenes (YD8-, YD10B-E6E7; lanes 3,5), but did not express E6 and E7 oncogenes (YD8-, YD10B-vector; lanes 2 and 4). The Caski cell line (lane 1) provided a positive control. The HPV16 E6E7 PCR products were electrophoresed in a 1.5% agarose gel and visualized under ultraviolet light by ethidium bromide staining. M, molecular marker (100 bp ladder).