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. 2013 May 31;43(2):375–382. doi: 10.3892/ijo.2013.1970

Figure 1.

Figure 1

Ly-294002 radiosensitizes CB193 and T98G. (A) Western blot analysis of AKT, AKT-P (phosphorylated form of AKT), PTEN and β-actin, 24 h after irradiation when CB193 and T98G were pre-treated with Ly-294002 or DMSO. (B and C) Cleaved caspase-3 detection by immunofluorescence 6 and 24 h after irradiation. Histograms showing the percentage of cleaved caspase-3-positive cells ± standard deviation with the respect to the total DAPI stained CB193 (B) and T98G (C) populations. Results are representative of two independent experiments (>400 cells analyzed per condition). (D) Colony forming unit (CFU) assay on CB193 and T98G treated with PI3K inhibitor (50 μM Ly294002) and irradiated with 2 or 5 Gy. A fixed number of living cells were seeded in plates with fresh culture medium without PI3K inhibitor 24 h after irradiation and colonies (>50 cells) were counted 14–20 days later. Mean number of colony forming unit from triplicate cultures ± standard deviation, are representative of two independent experiments. The curves were normalized to that of sham-irradiated control DMSO-treated cells. Statistics (t-test): *P<0.05; **P<0.01; ***P<0.001.