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SDS-PAGE analysis of p38α treated with 3 followed by “click” addition of Alexa594-azide: lane 1, p38α (2 μg) alone; lane 2. p38α (2 μg) subjected to “click” reagents; lane 3. p38α (0.4 μg) incubated with 3 followed by “click” reaction. (A): gel visualized by fluorescence. (B): Coomassie blue staining of the same gel.
