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Adduction of p38α by 3 is selectively blocked by recruitment site ligands. p38α was incubated in the presence of 3 (100 μM) alone (Control) or (A): with an equimolar concentration of the MKK3 D-site peptide (MKK3) or the Ste7 D-site peptide (Ste7) or (B) with an equimolar concentration of the ATP-binding site inhibitors SB 203580 or BIRB-796 for 16 h followed by click reaction and by in-gel fluorescence SDS-PAGE analysis.
