Figure 1. Cell fractionation, P-gp and BCRP basal expression.

(A) Cell fractionation: to verify proper fractionation of whole cell lysate, membrane fractions and cytosolic fractions of BeWo, Jar, MCF/P-gp cells were subjected to Western Blot analysis. Na⁺/K⁺ ATPase served as membrane marker, and NF-κB (p65 subunit) served as cytosolic marker. (B) P-gp and BCRP basal expression in MCF7/P-gp, Jar and BeWo cells was determined by Western Blotting. (C) Immunocytochemistry: fluorescent staining of BeWo, Jar and MCF7/P-gp cells with anti-P-gp antibody and DAPI. NC – negative control. (D) BCRP (ABCG2) expression in choriocarcinoma cell lines BeWo and Jar as demonstrated by FACS analysis.