Hydrogel properties over time. A) Young's modulus as determined by compression test of optimal-toughness IPN gels as a function of soaking time in cell culture conditions (n=3). No statistically significant (p<0.05) deviation in Young's modulus was detected by one-way ANOVA with a Tukey post-hoc test. B) IPN gel volume as a function of soaking time in cell culture conditions (n=3). No statistically significant (p<0.05) deviation in Young's modulus was detected by one-way ANOVA with a Tukey post-hoc test.