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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Virus Genes. 2012 Aug 4;45(3):508–517. doi: 10.1007/s11262-012-0793-y

Figure 1. Envelope expression constructs.

Figure 1

The JSRV Env expression constructs used in this study are shown. They were all based on a CMV-driven expression plasmid for JSRV, pCMV3JS21ΔGP (or ΔGP) (2), designated JSRV Envex in the figure. The constructs contain the R and U5 region from the upstream JSRV LTR, and the complete downstream LTR. The regions of Env encoding the SU and TM proteins are indicated. An equivalent construct expressing the Env protein of enJSRV 5F16 is designated JSRV Enven, and the endogenous Env sequences are shown as shaded boxes. For the other constructs, the following abbreviations are used: MSR, membrane spanning region of TM; AH, amphipathic helix in the cytoplasmic tail of TM; ED, ectodomain of TM. In all constructs shaded boxes indicate sequences derived from enJSRV 5F16, while clear boxes indicate sequences from JSRV. The relative sizes of the boxes in the TM protein are not to scale.