Table 1.
Effect of inhibitors on JSRV transformationa
| Transformed Foci | ||||||
|---|---|---|---|---|---|---|
| Exp 1 | Exp 2 | |||||
| FTI-277 | Rapamycin | FTI-277 | Rapamycin | |||
| pcDNA | 0 | 0 | 0 | 0 | 0 | 0 |
| JSRV Envex (ΔGP) | 98 | 50 | 29 | - | - | - |
| EnvexMSRen | 64 | 4 | 0 | - | - | - |
| Δ GP FLAG | - | - | - | 42 | 20 | 13 |
| EnvexMSRen FLAG | - | - | - | 13 | 2 | 0 |
a
RK3E cells were used in transformation assays of wild-type JSRV Env (ΔGP), EnvexMSRen (experiment 1), or FLAG epitope-tagged versions of the constructs (experiment 2) as described in Materials and Methods. pcDNA 3.1 (pcDNA), the empty expression vector, was used as a negative control. Assays were also performed in the presence of FTI-277 (5 µM) or Rapamycin (5 ng/ml) as shown. Numbers of foci arising are shown. RK3E cells were used in this assay because there is partial inhibition of ΔGP transformation by FTI-277, and the extent of inhibition by rapamycin is larger than in some other cells (16)
“-“, transfection with these plasmids was not performed.