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. 2013 Sep 17;11(9):e1001657. doi: 10.1371/journal.pbio.1001657

Figure 3. The RING-domain protein Roc1a, the adaptor protein SkpA, and the F-box protein Slimb are required for ddaC dendrite pruning.

Figure 3

(A–E′) Live confocal images of ddaC neurons expressing UAS-mCD8-GFP driven by ppk-Gal4 at WP and 16 h APF. Red arrowheads point to the ddaC somas, and purple arrowheads point to the ddaF soma also labelled by ppk-Gal4 (Chr II). While wild-type neurons eliminated dendrites (A, A′), roc1aG1 MARCM ddaC (B, B′), ddaC neurons overexpressing one copy of skpA RNAi #1 (C, C′) or two copies of skpA RNAi #2 (D, D′), or slimb8 MARCM (E, E′) ddaC clones exhibited dendrite pruning defects at 16 h APF. (F) Quantification of the average number of primary and secondary dendrites attached to the soma of wild-type and mutant ddaC neurons at WP stage and 16 h APF. The number of samples (n) in each group is shown on the bars. Error bars represent S.E.M. ***p<0.001, n.s., not significant. Dorsal is up in all images. The scale bars are 50 µm. See genotypes in Text S1.