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. 2013 Sep 17;11(9):e1001657. doi: 10.1371/journal.pbio.1001657

Figure 4. The Cul1-based SCF E3 ligase components interact with each other and cul1 expression is dependent on EcR-B1 and Sox14.

Figure 4

(A) Endogenous SkpA and Cul1 associated with Slimb in an ecdysone-independent manner. Nontreated (−) and ecdysone-treated S2 cells were transfected with Myc-Slimb. (B) Roc1a and Slimb associated in a complex with Cul1 and SkpA in S2 cells co-transfected with Myc-Slimb and Flag-Roc1a. (C) Endogenous SkpA and Cul1 co-immunoprecipitated with Myc-Slimb in the protein extracts from the wandering larval brains. (D) Expression profile of EcR-B1, sox14, mical, cul1, and uba1 in the MB γ neurons at eL3, WP, and 6 h APF. mRNA level of cul1, like sox14, mical, and uba1, was upregulated upon white prepupal formation. (E) Expression profile of EcR-B1, sox14, mical, cul1, and uba1 in MB γ neurons from wild-type or mutant animals at 6 h APF. mRNA level of cul1 was significantly reduced in EcRDN expressing or sox14Δ13 mutant MB γ neurons. **p<0.01, ***p<0.001, n.s., not significant. See genotypes in Text S1.