Skip to main content
. 2013 Sep 17;8(9):e74862. doi: 10.1371/journal.pone.0074862

Figure 1. Real time PCR expression analysis of genes putatively involved in drug transport across membranes in L. donovani isolates.

Figure 1

Expression ratios of resistant isolates (R1-R5) were relative to sensitive isolate S1 while laboratory resistant mutant Mt was compared with laboratory sensitive Dd8 strain. A. AQP1; B: MRPA. Results are mean of three independent experiments performed from three different RNA preparations. * P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005 indicate statistical significance with respect to reference sensitive strains S1 and Dd8 respectively; ns indicates no statistically significant difference. Inset shows the RT-PCR amplification curves; set a: curves for alpha tubulin amplification, set b: curves for MRPA / AQP1 (curve1: Dd8, 2: 144-R4, 3: Mt). X axis represents PCR cycle and Y-axis represents fluorescence.